Anti-Mouse H-2Db (Clone B22/249) – Purified in vivo PLATINUM™ Functional Grade

From

Price

1,650.00 ₪

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1.

H-2Db is a class I MHC cell surface protein.

MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface.

Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation.

For example, H-2Db plays a role in human papillomavirus infection2.

When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1.

B22-249.R1 was generated by immunizing mice with spleen cells from allogeneic mice using the combination BSLB/k anti-C57BL/63.

Spleen cells of the recipient mouse were hybridized with the P3-NS 1-Ag4 myeloma cell line and screened using cytotoxic and hemagglutination assays.

B22-249.R1 is also known as H-2.m2 in the literature4.

Package Size

Quantity

Storage and Stability

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Shipping Conditions

2-8°C Wet Ice