Anti-Mouse/Human CD49d (Clone PS/2) - Purified in vivo PLATINUM™ Functional Gr
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1,650.00 ₪
Integrins are a large family of heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation.
CD49d is a single-pass type I membrane glycoprotein also known as integrin alpha-4 (Uniprot Accession P13612).
CD49d is the α4 subunit of integrin heterodimers alpha-4/beta-1 (VLA-4; CD49d/CD29; α4β1 integrin) and alph-4/beta-7 (LPAM-1)1.
These integrins act as receptors for fibronectin and VCAM1 (CD106).
Integrin alpha-4/beta-7 is also a receptor for MADCAM1.
CD49d is expressed on most lymphocytes, granulocytes, monocytes, and thymocytes.
CD49d/CD29 (VLA-4; α4β1) is expressed at high levels on the surface of lymphohematopoietic progenitors and is involved in their development and proliferation.
CD49d/CD29 integrin/VCAM-1 interactions facilitate B cell adhesion to stromal cells and enhance B cell activation.
In the absence of alpha-4 integrins, pre-B cells fail to transmigrate and proliferate. PS/2 recognizes murine and human CD49d2.
PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0.
Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned.
Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells.
70Z/3 cells are also sensitive to PS/2 inhibition.
PS/2 is known to block binding of CD49d to its ligands3.
Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture2.
PS/2 is IgG2b κ.
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