Anti-Rat Kappa Light Chain - Purified in vivo PLATINUM™ Functional Grade

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1,650.00 ₪

MAR 18.5 is a monoclonal antibody directed against rat kappa light chains1.

MAR 18.5 was generated by immunizing SJL/J mice with soluble rat immunoglobulin, followed by the creation of a B cell hybridoma line via fusion of immune spleen with P3X63Ag8 myeloma cells.

MAR 18.5 hybridoma cells secrete an IgG2a kappa monoclonal antibody that strongly binds to protein A.

Additionally, MAR 18.5 antibody binds similarly to Ig of RI-1a and RI-1b allotypes.

MAR 18.5 antibody can be used in combination with anti-CD19 and anti-CD22 for in vivo B cell depletion in mice2,3.

In a study on Fcγ receptor-mediated phagocytosis, MAR 18.5 antibody was used as a secondary cross-linking antibody during stimulation of macrophages grown in medium lacking L cell–conditioned medium (LCM) and treated with chilled supernatant from the rat anti-FcγR 2.4G2 hybridoma4.

Additionally, MAR 18.5 antibody has been used for T cell isolation and complement lysis in combination with J11d.2 (anti-heat-stable Ag), 2.43 (anti-CD8), M5/114 (anti-class II), and 2.4G2 (anti-FcR)5.

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Storage and Stability

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Shipping Conditions

Next Day 2-8°C