Human Carbohydrate Sulfotransferase 4, CHST4 ELI

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands.

SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.

Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1.

Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody.

Plays a central role in lymphocyte trafficking during chronic inflammation.

Has a catalytic preference for core 2-branched mucin-type O-glycans.

Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors.

Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP.

Its substrate specificity may be influenced by its subcellular location.