Human E3 ubiquitin;ISG15 ligase TRIM25,TRIM25 ELI

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599).

Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1 (PubMed:17392790, PubMed:30193849).

Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712).

Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed:30193849).

Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755).

Mediates estrogen action in various target organs (PubMed:22452784).

Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).

Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity).

{ECO:0000250|UniProtKB:Q61510, ECO:0000269|PubMed:16352599, ECO:0000269|PubMed:17069755, ECO:0000269|PubMed:17392790, ECO:0000269|PubMed:22452784, ECO:0000269|PubMed:23950712, ECO:0000269|PubMed:30193849}.