Human Ectonucleoside Triphosphate Diphosphohydrolase 4, ENTPD4 ELI

Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines.

Preferentially hydrolyzes UTP and TTP.

AMP, ADP, ATP and UMP are not substrates (PubMed:10858452, PubMed:9556635). Preferentially activated by Ca2+ over Mg2+ (PubMed:10858452). Has a broad substrate specificity with the ability of cleaving all nucleotide di- and triphosphates with the exception of adenosine di- and triphosphate (ADP and ATP).

Preferentially hydrolyzes CTP, UDP, CDP, GTP and GDP.

Can use either Ca2+ or Mg2+ equally.